A dissociation binding experiment measures the off rate (koff) for ligand dissociating from the receptor. Initially, ligand and receptor were allowed to bind until equilibrium was reached. At that point, dissociation was initiated by massive introduction of unlabeled bromocriptine, a full agonist of dopamine D2 receptor (Figure 4). Binding decreased over time until it leveled off after overnight incubation. koff values were derived and were later used to calculate the kon rate.
Album · 2018 · 8 Songs. Keeping Me Right - Single. Sensitive Tip - Single. Past Full of Lessons - Single. Past Full of Chops & Screws - Single. Rhythm & Flow - Single. Sit Back & Vibe - EP.